Nonviral Mammalian Expression Systems

 

 

Transgene expression systems



Nonviral transgene

expression systems


Inducible expression

vector systems

miRNA expression vectors


Human miRNAs

and antagomirs



miRNA based
gene knockdown




Rat miRNAs

and antagomirs



Mouse miRNAs

and antagomirs




 

 

miRNA Expresion Vectors

 

Cellutron is providing you with most powerful miRNA tools to examine the effects of miRNAs on cellular function and phenotype. There are hundreds of inventoried vectors for overexpression of native miRNAs and antagomiR of your choice. Also, Cellutron has constructed expression vectors designed to express artificial miRNAs that are engineered to have 100% homology to your target gene and thus block gene expression.

 

Cellutron miRNA expression vectos allow allows you to:

1. Express artificial miRNA to knockdown the expression of target genes.

2. Transfect with high efficiency in variety of cell types including: dividing, non-diving cells, adult and embryonic stem cells and iPS cells.

3. Conditionally express miRNAs of your choice in vivo and in vitro.

4. Generate stable cell lines for long-term miRNA expression.

 

mirna cloning

 

 

General miRNA pathway

MicroRNAs (miRNAs) are small, 21- to 25-nucleotide-long, noncoding RNAs that regulate gene expression by base pairing with the 3′ untranslated regions and probably also with the coding sequences of target mRNAs, leading to translational repression or degradation.

 

miRNAs are transcribed by RNA polymerase II and can be derived from individual miRNA genes, from introns of protein-coding genes, or from polycistronic transcripts that often encode multiple, closely related miRNAs. Pri-miRNAs, generally several thousand bases long, are processed in the nucleus by the RNase Drosha into 70–100 nucleotide long hairpin-shaped precursors, called pre-miRNAs. Following transport to the cytoplasm, pre-miRNA is further processed by the RNA endonuclease Dicer to produce a double-stranded miRNA.


This miRNA duplex is then incorporated into a multicomponent protein complex known as RNA-induced silencing complex (RISC). During this process, one strand of the miRNA duplex is selected as the mature miRNA, while the other strand is in general rapidlyremoved and degraded. Nucleotides 2–8 of the 5′ end of the miRNA were named “seed sequence” because this region seems to be specifically important for mRNA target identification.


MiRNAs are well conserved in eukaryotic organisms and play a vital role in gene expression and regulation.



miRNA pathway

 

 

 

Welcome to CELLUTRON
 
 

 

 

 

USA Scientists

 

Fax: 1-800--994-6482

customerservice@cellutron.com

 

International scientists

 

Please fax over your purchase orders including following info:

 

1. Your name, tel and fax #

 

2. Your email address

 

3. Detailed shipping address.

 

4. Visa or MasterCard #, name on the card, expiration date and CVD

 

Fax: 1-410-238-2380

 

All Cellutron products should be used only in accordance with labels on the products and other products literature made available.
All Cellutron products are labeled “For Research Use Only”. Products are not approved for human or veterinary use or for application to humans.

 

year