Adult Rat Cardiomyocyte Isolation Steps

 

 

 
  • Perfusion Flow Rate:

  • A150 g - 600 g: 8 - 10 ml / min

 

 

The optimal tubing length should hold 12 ~ 13ml solution. Please adjust it before using our cell isolation system.

 

1. 2.
3. 4.

 

After perfusion, cut and digest the tissue as follows:

5. 6.
7. Perfusion 8. After perfusion, cut the tissue off from perfusion apparatus
9. Cut the tissue 10. Put the tissue pieces into 50 ml tube

11. Add 5 - 6 ml enzyme solution in the tube and shaking horizontally on a shaker .

12. Put the tissue pieces into 50 ml tube

Technical support:

techsupport@cellutron.com

 

                      BACK

 

 

 
   

 

 
 
Welcome to CELLUTRON
 

 

 

We construct vectors with

 

Your Genes

 

 

 

 

 

 

 

 

 

All Cellutron products should be used only in accordance with labels on the products and other products literature made available.
All Cellutron products are labeled “For Research Use Only”. Products are not approved for human or veterinary use or for application to humans.

 


year