Adult Rat Cardiomyocyte Isolation Steps



  • Perfusion Flow Rate:

  • A150 g - 600 g: 8 - 10 ml / min



The optimal tubing length should hold 12 ~ 13ml solution. Please adjust it before using our cell isolation system.


1. 2.
3. 4.


After perfusion, cut and digest the tissue as follows:

5. 6.
7. Perfusion 8. After perfusion, cut the tissue off from perfusion apparatus
9. Cut the tissue 10. Put the tissue pieces into 50 ml tube

11. Add 5 - 6 ml enzyme solution in the tube and shaking horizontally on a shaker .

12. Put the tissue pieces into 50 ml tube

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