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Adult Mouse Cardiomyocyte Perfusion
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Perfusion Flow Rate:
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The optimal tubing length should hold 12 ~ 13ml solution. Please adjust it before using our cell isolation system. |
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After perfusion, cut and digest the tissue as follows:
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1. Perfusion |
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2. After perfusion, cut the tissue off from perfusion apparatus
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3. Cut the tissue |
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4. Cut the tissue into small pieces
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5. Put the tissue pieces into 50 ml tube |
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6. Add 5 - 6 ml enzyme solution in the tube and shaking horizontally on a shaker . |
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For technical questions, please contact:
techsupport@cellutron.com
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7. Use 5 ml pipette with cut tip and pipet the tissue up and down |
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