Adult Mouse Cardiomyocyte Perfusion

 

 

 

Perfusion Flow Rate:

  • Adult mouse heart: 3 - 4 ml / min

     

The optimal tubing length should hold 12 ~ 13ml solution. Please adjust it before using our cell isolation system.

 

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After perfusion, cut and digest the tissue as follows:

         
 
   
 

1. Perfusion

   

2. After perfusion, cut the tissue off from perfusion apparatus

         
 
   
 

3. Cut the tissue

   

4. Cut the tissue into small pieces

         
 
   
 

5. Put the tissue pieces into 50 ml tube

   

6. Add 5 - 6 ml enzyme solution in the tube and shaking horizontally on a shaker .

         
 
 

For technical questions, please contact:

techsupport@cellutron.com

 

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7. Use 5 ml pipette with cut tip and pipet the tissue up and down

     
 
 

Cellutron Life Technologies

 

           

 

Cell Service

Cardiomyocyte isolation

Gene Service

Inducible miRNA expression

Minicircle Expression Vectors

miRNA expression vectors  

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

All Cellutron products should be used only in accordance with labels on the products and other products literature made available.
All Cellutron products are labeled “For Research Use Only”. Products are not approved for human or veterinary use or for application to humans.


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