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Minicircle Vectors for miRNA Expression

 

Cellutron provides high quality minicircle expression vectors containing transgenes, miRNAs and antigomirs of your interest (from species: human, mouse and rat).

 

Cat #	Vectors	100 ug	200 ug
m-8561	MC-CAG-GFP (negative control)	$ 495	$ 950
m-8562	MC-CAG-GFP-miRNA	$ 495	$ 950
m-8563	MC-CAG-RFP (negative control)	$ 495	$ 950
m-8564	MC-CAG-RFP-miRNA	$ 495	$ 950
m-8565	MC-CAG-miRNA (negative control)	$ 495	$ 950
m-8566	MC-CAG-miRNA	$ 495	$ 950

 

 

Advantages of Minicircle vector transfection

Compared to conventional plasmids, minicircle vectors have been demonstrated to greatly enhance transfection efficiency and to be capable of producing persistent high level of transgene expression in vitro and in vivo. Furthermore, minicircle DNA-based nonviral vector systems are advantageous over viral vector systems, such as much smaller size, better safety profile, no risk of insertional mutagenesis and no host immune and inflammatory response.

 

Vectors	Advantages	Disadvantages
Minicircle	Smaller DNA size (mainly, expression cassette) Higher transfection efficiency than plasmid DNA No silencing effect  on transgene expression caused by bacterial back bone Persistent  gene expression Suitable for in vitro and in vivo applications Not infectious  Essentially no limit to cloning capacity No integration Nonpathogenic No cytotoxicity No host immune response	N/A
Conventional plasmid	Not infectious No limit to the size of DNA No cytotoxicity	Low transfection efficiency Transient expression Silence of gene expression may occur Host immune response may occur Difficult for in vivo applications
Adenovirus	Highly effective in dividing and nondividing cells High transduction efficiency Essentially no integration	Transient expression Host immune response
Adeno-associated virus	Highly effective in dividing and nondividing cells Nonpathogenic Sustained gene expression Low immune response	Integration may occur Low loading capacity
lentivirus	Highly effective in dividing and nondividing cells High transduction efficiency Sustained gene expression Low immune response	Random integration Insertional mutagenesis
Retrovirus	Highly effective in dividing cells Sustained gene expression No cytotoxicity	Random integration Insertional mutagenesis