Nonviral Vector Systems
For Efficient Transgene Expression In Vitro and In Vivo
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Vector-based expression systems are invaluable tools for the study of gene function and its regulation, the development of various disease models, and clinical therapeutic applications. Although recombinant viruses have been widely used as vectors for gene transfer, there are major problems with inflammatory response, immunogenicity and insertional mutagenesis, which are still difficult to overcome despite extensive basic and clinical research. Therefore, nonviral vectors remain a viable alternative. Attention has now turned to plasmid vectors, which possess many advantages over viral vectors in terms of safety and ease of use. Many clinical studies have recently been performed using nonviral plasmid vector systems.
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